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Case 53

Hematopathology Case

CLINICAL INFORMATION

 

A 54-year-old male had an enlarged inguinal lymph node.

 

 

 

 

 

HE, 2

HE, 4

 

 

HE, 10

HE, 20

 

 

HE, 20

HE, 40

 

 

 

HE, 40

 

 

 

 

 

Flow Cytometry Assay

     Total events        A gate Bright CD45+ events  

     analyzed=20439      =59537 (86% of total nucleated cells)

        CD45+/CD14-          100%

        CD19+/CD3-           3%

        CD19-/CD3+           96%    

        TOTAL CD20+          3%

        CD19+/CD20+          3%

        CD19+/CD38+          3% 

        CD19+/CD10+         <2% 

        CD5+/CD19+          <2% 

        CD5+/CD19-          97% 

        CD19+/CD23+          3% 

        CD19+/FMC7+          3% 

        Total CD26+         97% 

        CD2+/CD7+           96% 

        CD3+/CD5+           97% 

        CD3+/CD4+           97%

        CD3+/CD8+           <2%

        Total CD16+         <2%

        CD3-/CD56+          <2%

           Gated on CD19+ cells

        CD19+/sIg Kappa+      65%

        CD19+/sIg Lambda+     34%

 

 

Click here for diagnosis and case discussion

 

Source: Department of Pathology, Creighton University Medical Center, Omaha, NE